brevican  (R&D Systems)

Journal: Experimental neurology

Article Title: Increased matrix metalloproteinase levels and perineuronal net proteolysis in the HIV-infected brain; relevance to altered neuronal population dynamics

doi: 10.1016/j.expneurol.2019.113077

Figure Lengend Snippet: MMPs cleave the perineuronal net proteins aggrecan and brevican. Active recombinant matrix metalloproteinases were incubated with recombinant aggrecan and brevican. In vitro digests revealed that A. aggrecan (Acan) and B. brevican (Bcan) are both cleaved by MMP-3 and MMP-13, as demonstrated by the appearance of the indicated cleavage fragments. Enzymatic cleavage is prevented by addition of the broad-spectrum MMP inhibitor GM6001.

Article Snippet: Recombinant human aggrecan (R&D, catalog # 1220-PG-025) was used at a concentration of 62.5 μg/mL and recombinant human brevican (R&D, catalog # 5800-NC-050) was used at a concentration of 50 μg/mL.

Techniques: Recombinant, Incubation, In Vitro

Journal: Journal of visualized experiments : JoVE

Article Title: Using microarrays to interrogate microenvironmental impact on cellular phenotypes in cancer.

doi: 10.3791/58957

Figure Lengend Snippet: Materials

Article Snippet: Brevican , R & D Systems , 4009-BC , ECM.

Techniques: Imaging, Inverted Microscopy, Microarray, Electron Microscopy