recombinant human brevican  (R&D Systems)

Journal: Experimental neurology

Article Title: Increased matrix metalloproteinase levels and perineuronal net proteolysis in the HIV-infected brain; relevance to altered neuronal population dynamics

doi: 10.1016/j.expneurol.2019.113077

Figure Lengend Snippet: MMPs cleave the perineuronal net proteins aggrecan and brevican. Active recombinant matrix metalloproteinases were incubated with recombinant aggrecan and brevican. In vitro digests revealed that A. aggrecan (Acan) and B. brevican (Bcan) are both cleaved by MMP-3 and MMP-13, as demonstrated by the appearance of the indicated cleavage fragments. Enzymatic cleavage is prevented by addition of the broad-spectrum MMP inhibitor GM6001.

Article Snippet: Recombinant human aggrecan (R&D, catalog # 1220-PG-025) was used at a concentration of 62.5 μg/mL and recombinant human brevican (R&D, catalog # 5800-NC-050) was used at a concentration of 50 μg/mL.

Techniques: Recombinant, Incubation, In Vitro

Journal: Experimental neurology

Article Title: Increased matrix metalloproteinase levels and perineuronal net proteolysis in the HIV-infected brain; relevance to altered neuronal population dynamics

doi: 10.1016/j.expneurol.2019.113077

Figure Lengend Snippet: Perineuronal net protein levels are altered in HIV-infected human brain tissue. Aggrecan and brevican were detected by Western blot using post-mortem human cortical lysates. Representative bands from infected and non-infected shown. A. Levels of the aggrecan (Acan) cleavage fragment (27 kDa) were elevated in HIV-infected samples (unpaired t test, df = 36, t = 2.086, p = 0.0441). B. Levels of intact full-length brevican (Bcan) (160 kDa) were decreased in HIV-infected samples (unpaired t test, df = 36, t = 5.985, p < 0.0001). C. Correlations between MMPs and PNN protein expression were assessed. MMP-3 levels were positively correlated with levels of aggrecan cleavage fragments (Pearson correlation; r = 0.4023, R2 = 0.1618, p = 0.0150, n = 36), and D. negatively correlated with full-length brevican (Pearson correlation; r = −0.4450, R2 = 0.1980, p = 0.0065, n = 36). E. There was a significant positive correlation between levels of MMP-13 and the aggrecan cleavage fragment (Pearson correlation; r = 0.9301, r2 = 0.8651, p < 0.0001, n = 36), and F. there was a trending negative correlation between levels of MMP-13 and brevican (Pearson correlation; r = −0.2928, r2 = 0.08575, p = 0.0831, n = 36). HIV-negative (black arrows) n = 15, HIV-positive (purple circles) n = 21; for all correlations the best fit line and 95% confidence bands were generated using linear regression. * p < 0.05, **** p < 0.0001.

Article Snippet: Recombinant human aggrecan (R&D, catalog # 1220-PG-025) was used at a concentration of 62.5 μg/mL and recombinant human brevican (R&D, catalog # 5800-NC-050) was used at a concentration of 50 μg/mL.

Techniques: Infection, Western Blot, Expressing, Generated

Journal: Journal of visualized experiments : JoVE

Article Title: Using microarrays to interrogate microenvironmental impact on cellular phenotypes in cancer.

doi: 10.3791/58957

Figure Lengend Snippet: Materials

Article Snippet: Brevican , R & D Systems , 4009-BC , ECM.

Techniques: Imaging, Inverted Microscopy, Microarray, Electron Microscopy